Table 1. Extracellular matrix does not affect bile acid uptake in hepatocytes.
Hepatocytes plated on the indicated ECM were exposed to radiolabeled bile acids 14C–glycocholate and 3H–taurocholate for 30 minutes. Cellular uptake was quantified via liquid scintigraphy and expressed as nmol/mg protein. Results represent mean ± standard deviation of 3 separate experiments.
| Uptake nmol/mg protein | ||
|---|---|---|
| ECM | TC (50 uM) | GC (50 uM) |
| Type I Collagen | 19.0 (4.9) | 16.1 (1.9) |
| Type IV Collagen | 18.7 (4.2) | 16.2 (2.3) |
| Fibronectin | 19.3 (3.6) | 17.0 (1.6) |
| Laminin | 21.4 (4.3) | 19.1 (3.2) |
| Polylysine | 20.0 (3.8) | 18.1 (3.2) |