Table 1. Inhibition of insulin release from rat pancreatic islets by inhibitors of α-ketoglutarate hydroxylases.

Islets were incubated in the presence of the secretagogues for 1 h. Inhibitors were added 10 min before the secretagogues. The concentration of glucose was 16.7 mM and the concentrations of leucine and glutamine were 10 mM. Results are the mean ± SE (n = 6 to 12 replicate incubations for each condition)

Secretagogue	Inhibitor	Insulin Release(μU Insulin/5 islets/ 60 min.)
None	None	20 ± 2
Glucose	None	841 ± 63
Glucose	1 mM Ethyl-3,4-dihydroxybenzoate	48 ± 5^a
Glucose	10 mM 2-Methyl-α-ketoglutarate	54 ± 7^a
Glucose	5 mM 2-Methyl-α-ketoglutarate	291 ± 21^a
Glucose	10 mM 3-Methyl-α-ketoglutarate	315 ± 29a
Leucine + glutamine	None	1094 ± 105
Leucine + glutamine	1 mM Ethyl-3,4-dihydroxybenzoate	72 ± 24^a

p < 0.001 vs same secretagogue condition without inhibitor