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. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: Biochimie. 2008 Feb 29;90(8):1149–1171. doi: 10.1016/j.biochi.2008.02.020

(A) Structures of the G-quadruplex-interactive compounds TMPyP4, telomestatin, and Se2SAP, and the control compound TMPyP2. (B) The Taq polymerase stop assay was used to compare the stabilization of the PDGF-A G-quadruplexes by TMPyP2 (lanes 3–7), TMPyP4 (lanes 8–12), telomestatin (lanes 13–17), and Se2SAP (lanes 18–22) by using increasing concentrations of drugs (0.01, 0.05, 0.5, 1, and 2.5 µM) at 60 °C. Lane 1 is control and lane 2 is without drug. (C) The ratios of the major arrest products of each sample to the total product were plotted against drug concentrations. (D) Dual luciferase assay to determine the effect of TMPyP4 and TMPyP2 on the transcriptional activity of PDGF-A basal promoter containing the NHE. The comparative firefly luciferase expressions (firefly/renilla) of TMPyP2 and TMPyP4 are shown in the histograms [56].

(A) Structures of the G-quadruplex-interactive compounds TMPyP4, telomestatin, and Se2SAP, and the control compound TMPyP2. (B) The Taq polymerase stop assay was used to compare the stabilization of the PDGF-A G-quadruplexes by TMPyP2 (lanes 3–7), TMPyP4 (lanes 8–12), telomestatin (lanes 13–17), and Se2SAP (lanes 18–22) by using increasing concentrations of drugs (0.01, 0.05, 0.5, 1, and 2.5 µM) at 60 °C. Lane 1 is control and lane 2 is without drug. (C) The ratios of the major arrest products of each sample to the total product were plotted against drug concentrations. (D) Dual luciferase assay to determine the effect of TMPyP4 and TMPyP2 on the transcriptional activity of PDGF-A basal promoter containing the NHE. The comparative firefly luciferase expressions (firefly/renilla) of TMPyP2 and TMPyP4 are shown in the histograms [56].