FIG. 5.

The effect of DSI spike frequency on C2 spike-evoked Ca²⁺ signaling and synaptic strength. A: calcium signals in C2 as a function of DSI spike frequency. DSI was stimulated for 5 s at various spike frequencies. Ca²⁺ signals are expressed as percentage pretest. *, significant differences from C2 stimulated with no DSI DSI stimulation (0 Hz) with a Friedman repeated-measures ANOVA on ranks (performed using normalized data, with the factor being the level of DSI stimulation) with post hoc pair-wise comparisons (Student-Newman-Keuls, n = 8). B: C2-evoked EPSPs in postsynaptic pedal neurons plotted as a function of DSI stimulation. *, significant differences with a 1-way repeated-measures ANOVA [performed using normalized data, with the factor being the level of DSI stimulation (0, 5, 10, 20 Hz) with post hoc pair-wise comparisons (Student-Newman-Keuls, F = 15.515, df between subjects = 7, df between treatments = 3, n = 8)].