Table 1.
ERG27 mutations obtained as a result of site-directed mutagenesis (SDM) or random mutagenesis (RM) correlated with sterol profiles and predicted site of lesion
| Type of sterol profile | SDM or RM | Predicted site of lesion in Erg27p | |
|---|---|---|---|
| Group I | High 3-keto sterone accumulation | ||
| Y202F | SDM | Active site | |
| K206A | SDM | Active site | |
| N153L | SDM | Active site | |
| R40A | SDM | SDR conserved site | |
| G10A | SDM | NADPH binding site | |
| D75G/N153D | RM | ||
| S183P/F235S | RM | ||
| S205P/S237G | RM | ||
| Group II | High squalene epoxide accumulation | ||
| L212P | RM | ||
| S180C | RM | ||
| L23P | RM | ||
| S205P/S237G | RM | ||
| L42P/D68G/A152T | RM | ||
| Group III | Intermediate sterol accumulation | ||
| Q311*1 | RM | ||
| P232A | SDM | SDR conserved site | |
| R32K/S239P | RM | ||
| I135V/L148P | RM | ||
| P166L/S179L | RM | ||
| Q46R/D54G/N146V/K206E | RM | ||
| Wildtype-like sterol accumulation | |||
| Group IV | T9A | ||
| S13A | SDM | NADPH binding site | |
| D75A | SDM | NADPH binding site | |
| N84S | SDM | SDR conserved site | |
| I111M | RM | ||
| V140A | RM | ||
| F150L | RM | ||
| M182V | RM | ||
| S189A | RM | ||
| D314A | SDM | Active site | |
| K337E | SDM | SDR conserved site | |
| T26I/F160L | RM | ||
| F246A/F247A | RM | ||
| SDM | SDR conserved site |
1
Q311* results from a stop codon at amino acid 311. Slashes indicate multiple amino acid substitutions