Fig. 5. DiI-stained neurons transplanted to a cultured slice.

Stained embryonic hippocampal neurons were plated onto cultured hippocampal slices as described in Materials and Methods. This image was made 6 days after plating, using the TPLSM at 960 nm, from a z-series of 30 scans, 2 um apart. No photobleaching or phototoxicity was detectable during or after scanning.