Figure FIG. 1.

Mutational analyses of non‐clonal and clonal cultures of BMSCs derived from fibrous dysplastic lesions. Non‐clonal cultures were analyzed by selective amplification of the mutated allele using a PNA primer to block amplification of the normal allele (which detects 1:200 mutated cells) and by direct DNA sequencing (which detects 1:2 or 3 mutated cells) to approximate the mutational load within the BMSC population. Clonal cultures were established to determine the colony forming efficiency (the number of CFU‐Fs), and isolated clones were analyzed by direct DNA sequencing to determine the number of mutated clones.