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. Author manuscript; available in PMC: 2008 Nov 20.
Published in final edited form as: Hum Mol Genet. 2002 Oct 15;11(22):2723–2733. doi: 10.1093/hmg/11.22.2723

Figure 3.

Figure 3

Co-immunoprecipitation of endogenous NUB1 with FLAG–AIPL1. Empty vector (lane 1) or plasmid DNA encoding FLAG-tagged AIPL1 (lane 2), p53 (lane 3), HHR23 (lane 4) or RanGAP1 (lane 5) was overexpressed in COS cells. The total cell lysate was analyzed by antibody against FLAG (top panel) or NUB1 (middle panel). The total cell lysate was also incubated with anti-FLAG antibody for immunoprecipitation. Co-precipitated proteins were analyzed by immunoblotting using anti-NUB1 antibody (bottom panel). Only FLAG-tagged AIPL1 is capable of immunoprecipitating NUB1 (bottom panel, lane2), thereby demonstrating that these two proteins interact in vitro and in vivo. Molecular mass standards are expressed in kilodaltons.