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Co-immunoprecipitation of NUB1 with AIPL1 in human Y79 retinoblastoma cells. Immunoprecipitates with preimmune serum (lane 1), anti-AIPL1 serum (lane 2) and affinity-purified anti-AIPL1 antibody (lane 3) were separated by SDS–PAGE and transferred for immunoblot analysis (IB) with either anti-NUB1 (A) or unpurified anti-AIPLl (B). Total Y79 cell lysate (lane 4) was also loaded on the gel.
