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. Author manuscript; available in PMC: 2009 Jul 23.
Published in final edited form as: FEBS Lett. 2008 Jun 20;582(17):2508–2514. doi: 10.1016/j.febslet.2008.06.016

Figure 5. Inhibiting PDGFR simultaneously with RA induced differentiation enhances endothelium mobility.

Figure 5

Figure 5

HL-60 cells were treated with RA in absence or presence of 10 µM AG1296 for 72 h. A total of 2 million HL-60 cells in 2 ml of fresh media were pipetted on a monolayer of EAhy926 endothelial cells that were grown in a 6 well plate for 72h, wounded with a pipette tip and washed extensively with PBS prior to addition of HL-60 cells. Following 24h of co-culture, the cell suspension was removed, the endothelial layer was washed with PBS at 37C. One milliliter of the warm PBS was added to the wells in order to take the pictures. Figure 5A shows endothelial cell migration, in a representative experiment, after exposure to control, AG1296, RA, or RA plus AG1296 treated HL-60 and the figure 5B presents the quantization resulting from 3 independent experiments.