Table 2.
Identified Cross-Linked Proteins from > 250 kD Gel Bands in Anti-OmcA and Anti-MtrC IP of MRN and DSS in Vivo Labeling on MtrC and OmcA Mutant Cells, Respectivelya
anti-OmcA |
anti-MtrC |
||||||
---|---|---|---|---|---|---|---|
ΔMtrC |
ΔOmcA |
MW |
|||||
gene | MRN | DSS | MRN | DSS | annotation | categoryb | (kDa) |
SO1779 | 971 | 476 | decaheme cytochrome c, OmcA | OM | 79 | ||
SO1778 | 333 | 312 | decaheme cytochrome c, MtrC | OM | 72 | ||
SOa0110 | 308 | lipoprotein, putative | OM | 145 | |||
SO3545 | 472 | 149 | OmpA family protein | OM | 40 | ||
SO3896 | 100 | 125 | outer membrane porin, putative | OM | 39 | ||
SO1295 | 183 | 153 | 179 | 106 | major outer membrane lipoprotein | OM | 9 |
SO1824 | 69 | conserved hypothetical | P | 25 | |||
SO3286 | 68 | cytochrome c ubiquinol oxidase | IM | 58 | |||
SO1490 | 306 | alcohol dehydrogenase II, AdhB | C | 40 | |||
SO4747 | 108 | ATP synthase F1, beta subunit | C | 49 | |||
SO0217 | 82 | 179 | translation elongation factor Tu | C | 43 | ||
SO0237 | 105 | ribosomal protein S3 | C | 25 | |||
SO0314 | 57 | ornithine decarboxylase | C | 82 |
The numbers in the table are the Mascot scores.
OM denotes outer membrane; P denotes periplasmic; IM denotes inner membrane; and C denotes cytoplasmic. ΔMtrC denotes mtrC mutant, and ΔOmcA denotes omcA mutant.