Table 3.
Identified Cross-Linked Proteins from >250 kD Gel Bands in Anti-OmcA IP of MRN and DSS Cell-Lysate Labelinga
gene | DSS | MRN | annotation | categoryb | MW (kDa) |
---|---|---|---|---|---|
SO0225 | 1928 | DNA-directed RNA polymerase, rpoC | C | 156 | |
SO0224 | 1431 | 74 | DNA-directed RNA polymerase, rpoB | C | 150 |
SO1490 | 987 | alcohol dehydrogenase II, AdhB | C | 40 | |
SO0229 | 928 | 359 | translation elongation factor Tu, TufA | C | 43 |
SO0217 | 888c | 367c | translation elongation factor Tu, TufB | C | 43 |
SO4749 | 467 | ATP synthase F1, alpha subunit | C | 55 | |
SO1142 | 321 | 88 | carbamoyl-phosphate synthase, CarB | C | 119 |
SO4747 | 238 | 45c | ATP synthase F1, beta subunit | C | 50 |
SO2638 | 170 | leucine dehydrogenase, Ldh | C | 37 | |
SO0314 | 161 | 130 | ornithine decarboxylase, SpeF | C | 82 |
SO3032 | 160 | NTP-dependent alcaligin synthetase, AlcC | C | 69 | |
SO1679 | 120 | branched-chain acyl-CoA dehydrogenase | C | 42 | |
SOA0112 | 117 | lipoprotein, putative | OM | 146 | |
SO1521 | 100 | conserved hypothetical protein | C | 103 | |
SO3532 | 91 | isoleucyl-tRNA synthetase, IleS | C | 106 | |
SO0021 | 86 | fatty oxidation complex, alpha subunit | C | 77 | |
SO0256 | 81 | DNA-directed RNA polymerase, RpoA | C | 36 | |
SO4030 | 73 | excinuclease ABC, A subunit, UvrA | C | 106 | |
SO1295 | 68c | 138c | major outer membrane lipoprotein | OM | 9 |
SO3440 | 56 | enolase, Eno | C | 42 | |
SO1779 | 41c | 140c | OmcA | OM | 80 |
SO3542 | 55 | conserved hypothetical protein | C | 90 |
The numbers in the table are the Mascot scores.
C denotes cytoplasmic, and OM denotes outer membrane.
Indicates that those proteins were also identified from on-cell labeling.