Fig. 3.

Cocaine induced locomotion and sensitization in A-FOS mice. On five consecutive days, A-FOS expressing and control mice were treated with (A) saline, (B) 5 mg/kg or (C) 10 mg/kg cocaine, immediately placed in a novel environment consisting of a 30×30 cm box and their locomotion measured for 15 minutes using a video tracking system. The graphs display the group means (n=11 to 13 each) and standard errors of total distance traveled for fifteen minutes over the five treatment days. **=p<0.01 and *=p<0.05 relative to day 1 (Bonferroni post-tests). (D) Following six days without treatment, all three groups of animals were tested with a single injection of 10 mg/kg cocaine and immediately placed into the same 30×30 box and their locomotion tracked. The graph displays the group means and standard errors of total distance travel in cm. # significant effect of dose p<0.05 as determined by 2-way-ANOVA. When the control and A-FOS groups were analyzed separately by 1-way ANOVA, the 5mg and 10mg pretreated animals from each group had significantly great (p<0.05) locomotion relative to their respective saline treated controls. *=p<0.05 relative to controls at the same dose. (Bonferroni Post-Test ). +=p<0.05 relative to saline treated animals the same group, control or A-FOS.