Table 2.

Confirmation of a random sample of genes identified by microarrays as differentially expressed. 10 genes from the A-FOS regulated gene list and 6 genes from the cocaine regulated gene list were randomly picked and qPCR used to confirm their differential expression in the striatum from an independent group of animals treated identically to the animals used in the microarray studies. Additionally 3 genes identified to be regulated by these conditions were also tested; Bdnf, Egr1, Fos. The ΔCt value for each gene was determined and a standard T-test was used to identify differences in expression (*= p<0.05). 88% of the randomly chosen genes were confirmed differentially expressed. Table 2 lists the genes using the official gene symbol along with the expression difference based on changes in ΔCt (ΔΔCt) relative to the control or untreated group. A negative number indicates an increase in expression in the A-FOS or cocaine treated animal.

Gene Symbol	ΔΔCt
A-FOS regulated
Bdnf	−2.24	*
Clk1	0.66
Ctsb	−2.29	*
Eif4b	1.28	*
Entpd3	2.47	*
Fundc1	3.84	*
Gpr123	−2.05	*
Homer2	−3.21	*
Odz4	−0.36
Per1	1.74	*
Sbsn	1.78	*
Cocaine regulated
BB471698	−2.38	*
Camk4	1.81	*
Egr1	−3.08	*
Fos	−2.18	*
Gnb5	2.00	*
Hspa1a	−1.83	*
Pde10a	−1.29	*
Plcb1	3.02	*