Table 2.

Dissociation constants of HTPs from thiazole orange displacement.

HTP	Dissociation constants (FID) (M)[a]
13	1.4 ± 0.3
14	> 30
15	6.2 ± 0.6
16	18.9 ± 4.1
17*	10.1 ± 1.8
18	13.4 ± 4.2
19	0.35 ± 0.08
20	3.9 ± 0.3
21	3.6 ± 1.9
22	14.2 ± 1.6
23	> 30
24	> 30

^[a]Conditions: 50 mM Bis-Tris·HCl, pH 7.0, 100 mM NaCl, 10 mM MgCl₂, 50 nM thiazole orange and 100 nM RNA A, 25 °C. Dissociation constants are reported as the average of three independent measurements ± standard deviation.

^*Average of two independent measurements.