Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 7;9:66. doi: 10.1186/1471-2172-9-66

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008 Zhonghua et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Expression analysis of the BS CXC. (A) The expression of BSCXC mRNA relative to b-actin analyzed by real-time PCR in Black Seabream after PolyI:C and LPS challenge. The values are shown as means ± S.E., n = 3. Significant differences between challenged group and control group are indicated by an asterisk (P < 0.01). (B) The plasmid BS CXC-pQE30 was transformed into E. coli M15 cells and induced by ITPG. Bacterial lysates (lane 1) or purified BS rCXC (lanes 3 and 4) were separated on SDS-PAGE and stained by Coomassie brilliant blue R250. Lysate from uninduced cells was used as a control (lane 2). (C) The identity of the BS rCXC was confirmed by Western blotting using the antibody of 6His. Lanes 1 and 2 showed BS rCXC.