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Schematic map of vector construction. Minigenes encoding for human or mouse gp10025–33 and TRP-2181–188 were assembled by PCR with overlapping oligonucleotides as templates. The PCR fragments generated were cloned into pCMV-Ub (13) by using a unique BglII restriction site downstream of the coding sequence for mouse ubiquitin. SV40, simian virus 40.
