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. 2000 Apr 25;97(10):5492–5497. doi: 10.1073/pnas.090097697

Figure 3.

Figure 3

Induction of tumor-protective immunity. C57BL/6J mice (n = 8) received three vaccinations of 108 attenuated S. typhimurium (SL7207) harboring DNA vaccines by oral gavage at 2-week intervals. Experimental groups were immunized with vaccines encoding ubiquitinated murine gp10025–33 and TRP-2181–188 peptide epitopes (pUb-M), ubiquitinated human gp10025–33 and TRP-2181–188 epitopes (pUb-H), or the murine peptide epitopes without ubiquitin (p-Mini). Control groups of mice (n = 8) included those treated with PBS or a vaccine containing only the empty vector (pUb-V). Mice were challenged with 1 × 105 B16G3.26 murine melanoma cells 1 week after the final vaccination. Bars represent means and standard deviations of eight mice per group. Differences in tumor volume between experimental groups treated with either pUb-M or pUb-H and all control groups were statistically significant (P < 0.01).