Figure 6.

Cytokine release by CD8⁺ T cells isolated from splenocytes of C57BL/6J mice treated with DNA vaccines. Release of IFN-γ by CD8⁺ T cells isolated from splenocytes of C57BL/6J mice was measured by solid-phase sandwich ELISA. Splenocytes were obtained from mice immunized with either pUb-H or pUb-M DNA vaccines and from control mice treated with either PBS or the empty ubiquitin vector (pUb-V). Bars represent means and standard deviations of eight mice per group. Differences in IFN-γ release observed between experimental groups treated with either pUb-M or pUb-H and all control groups were statistically significant (P < 0.01).