Table 1.
GFP-Stau and oskMS2/MS2-GFP Particle Behavior in Localization Mutants
Phenotype | Number of Tracks | Number of Movies | Track Distance, μm | Average Speed, μm/s | Percent Tracks to Anterior | Percent Tracks to Posterior | Net Posterior Displacement, μm/s |
---|---|---|---|---|---|---|---|
WT (GFP-Stau) | 491 | 28 | 2.43 (0.07) | 0.45 (0.01) | 43.6 | 56.4 | +0.03 (0.01) |
btz2 (GFP-Stau) | 77 | 20 | 2.88 (0.19) | 0.53 (0.03) | 57.1 | 42.9 | −0.02 (0.04) |
TmIIgs (GFP-Stau) | 150 | 23 | 1.83 (0.09) | 0.77 (0.03)∗∗∗ | 58.7 | 41.3 | −0.12 (0.04)∗∗∗ |
mago (GFP-Stau) | 192 | 30 | 1.84 (0.1) | 0.62 (0.02)∗∗∗ | 58.9 | 41.1 | −0.057 (0.03)∗∗∗ |
WT (oskMS2/MS2-GFP) | 272 | 9 | 2.85 (0.11) | 0.47 (0.01) | 43.0 | 57.0 | +0.04 (0.02) |
TmIIgs(oskMS2/MS2-GFP) | 199 | 17 | 2.09 (0.08) | 0.55 (0.02)∗∗ | 58.8 | 41.2 | −0.08 (0.03)∗∗ |
stau (oskMS2/MS2-GFP) | 152 | 31 | 3.00 (0.1) | 0.47 (0.01) | 40.8 | 59.2 | +0.08 (0.03) |
Khc27 (oskMS2/MS2-GFP) | 154 | 19 | 2.37 (0.09) | 0.50 (0.02) | 49.4 | 50.6 | +0.02 (0.03) |
Khc17 (oskMS2/MS2-GFP) | 272 | 8 | 2.59 (0.09) | 0.35 (0.01) ∗∗∗ | 43.8 | 56.2 | +0.01 (0.01) |
Khc23 (oskMS2/MS2-GFP) | 231 | 8 | 3.01 (0.11) | 0.25 (0.004)∗∗∗ | 47.2 | 52.8 | +0.004 (0.01)∗ |
Dhc6-6/6-12 (oskMS2/MS2-GFP) | 231 | 21 | 3.06 (0.11) | 0.53 (0.01)∗∗∗ | 42.7 | 57.3 | +0.04 (0.02) |
Values shown are means for all particles that move faster than cytoplasmic flows (>0.1 μm/s) with the SEM in the parentheses. Statistically significant differences between the behavior of particles in wild-type and a given mutant are shown by asterisks: ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001. Corresponding p values are shown in parentheses. WT: wild-type.