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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2008 Aug 8;232(3):469–477. doi: 10.1016/j.taap.2008.08.001

Figure 7.

Figure 7

Assocation of IL-8 with diesel soot is reversible. SRM2975/radiolabeled IL-8 mixtures were incubated for 2 h. The particles were removed by centrifugation, washed twice in PBS with 0.01% Tween-80, and resuspended in the same buffer, alone (open squares, heavy line) or in the presence of BSA at 1 mg/ml (open circles, dotted line) or 10 mg/ml (solid circles, thin line), or 50 μg/ml cytochrome C (solid triangles, dashed line). At intervals thereafter, suspensions were centrifuged and the label released to the supernatant was determined. The fraction remaining bound was plotted as a function of time. Curves are the best fit to a single exponential decay model. Data shown are from one of three similar experiments. Error bars represent the SEM of duplicate measurements.