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. Author manuscript; available in PMC: 2009 Oct 31.
Published in final edited form as: Cell. 2008 Oct 31;135(3):535–548. doi: 10.1016/j.cell.2008.09.057

Figure 4.

Figure 4

MyoVb Constitutively Traffics REs in Spines by Interacting with Rab11-FIP2

(A) Hippocampal neurons expressing GFP-MyoVb WT, ΔRBD, or CCtr were stained for endogenous Rab11-FIP2. The dendritic outline was determined by a mCherry cell fill. Colocalization is indicated by white arrows. Scale bar, 2 μm.

(B) Pixel-by-pixel fluorescence intensity correlations between GFP-MyoVb-WT, ΔRBD, or CCtr with endogenous Rab11-FIP2. Correlation coefficients (CC) are indicated. AFU, arbitrary fluorescence units.

(C) Hippocampal neurons expressing TfR-mCh, GFP, and V5-tagged MyoVb WT, ΔRBD or CCtr were stained for V5. The dendritic outline was determined by GFP. Scale bar, 2 μm.

(D) Quantitative analysis of the normalized fraction of MyoVb associated with REs. Data represent means ± SEM. **p <0.001 relative to WT; t-test.

(E) Quantitative analysis of RE mobility in spines over time. Neurons expressing TfR-mCh and GFP-MyoVb WT or mutants were imaged for 5 min at 37°C. The normalized TfR-mCh fluorescence (F/F0) in 30 dendritic spines for each construct was plotted over time.

(F) Coefficient of variance (CV) of the normalized intensity of TfR-mCh in spines over a 5 min time lapse. Data represent means ± SEM of the CV. *p<0.01 relative to WT; t-test.