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. Author manuscript; available in PMC: 2009 Oct 31.
Published in final edited form as: Cell. 2008 Oct 31;135(3):535–548. doi: 10.1016/j.cell.2008.09.057

Figure 5.

Figure 5

MyoVb Mediates RE Translocation into Spines Following LTP-Inducing Stimuli

(A-C) Time lapse images of TfR-mCh and GFP-MyoVb-WT (A), ΔRBD (B), or CCtr (C) in a dendritic spine. Two time points before and a six minute time lapse after glycine (black arrow) are shown. Red arrows indicate co-transport of GFP-MyoVb WT or CCtr and REs following glycine stimulation. Green arrows indicate the lack of correlated movement between GFP-MyoVb ΔRBD and RE. Time is indicated in min:sec. See Movies S6-S8. Scale bar, 2 μm.

(D-F) Cumulative probability plots of GFP-MyoVb WT (D), ΔRBD (E), and CCtr (F) fluorescence intensity on REs before and 2.5 min after glycine. The ΔRBD mutant blocks, while the CCtr mutant occludes the glycine-induced association of MyoVb with REs.

(G) Data indicate means ± SEM of the fold increase in TfR-mCh fluorescence intensity 10-15 min after glycine in spines initially lacking REs. **p<0.001; t-test.