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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Dev Biol. 2008 Mar 18;318(1):17–28. doi: 10.1016/j.ydbio.2008.02.050

Fig. 4.

Fig. 4

D6 is required for Nkd function and nuclear localization. (A) nkd da>NkdGFP rescue and localization as in Fig. 1A. (B) Distribution of wild type and nkd cuticle phenotypes for rescue by indicated constructs as in Fig. 1B. (C) Rescue of elevated Arm in alternate segments of nkd mutants via prd-Gal4 (nkd prd>) as in Fig. 1C (ordinate axis 0–250 pixels). (D–I) 71B>NkdGFP third instar salivary gland imaged for GFP. NkdΔD6/GFPC (D) is in perinuclear aggregates (arrowhead) but has reduced nuclear localization (arrow) compared to NkdGPFC in Fig. 3A. NkdΔ30aa/GFPC (E) localizes to cytoplasm and nucleus in a distribution very similar to NkdGFPC, but NkdΔD6Δ30aa/GFPC (F) is excluded from nuclei. NkdΔR1S/GFPC (G) is almost exclusively nuclear, with enrichment at the nuclear membrane (arrow). NkdΔR1SΔD6/GFPC (H) exhibits reduced nuclear localization relative to NkdΔR1S/GFPC, while NkdΔR1SΔD6Δ30aa/GFPC (I) is excluded from the nucleus.