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Antibacterial activity of SgI and SgII holoproteins requires cleavage by PSA. A. Seminal plasma (SP), purified SgI, purified SgII, and PSA were run on an AU-PAGE gel and analyzed by coomassie staining or by antibacterial gel overlay assay with E. coli.
B and C. SgI was cleaved by PSA for 4h (B) or for 24h (C) and run on an AU-PAGE gel and analyzed by coomassie staining or by antibacterial gel overlay assay with E. coli.
