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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Immunol. 2008 Sep 1;181(5):3413–3421. doi: 10.4049/jimmunol.181.5.3413

Figure 8.

Figure 8

Antibacterial activity of semenogelin-depleted seminal plasma. Semenogelin was depleted from seminal plasma by applying divalent cation-depleted seminal plasma to nickel agarose and the semenogelin was eluted with imidazole. The imidazole was removed by dialysis and zinc was added both to the semenogelin depleted seminal plasma and to the semenogelin containing eluate. Normal seminal plasma (SP), flow through from the nickel agarose representing the semenogelin-depleted seminal plasma and the eluate representing the semenogelin-free seminal plasma were run on a AU-PAGE gel and subjected to immunoblotting with anti-semenogelin antibodies and anti-SLPI antibodies (A) or analyzed in a gel overlay assay against E. coli (B). 2D gel analysis of the cationic fraction of the semenogelin eluate (C). Proteins were identified by spot matching. I denotes SgI and II denotes SgII.