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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: Cancer Res. 2008 Aug 15;68(16):6810–6821. doi: 10.1158/0008-5472.CAN-08-0141

Figure 5.

Figure 5

Effect of hMOF downregulation on TMS1 DNA methylation. A) MCF7 cells were transfected with siRNA (100 nM) against hMOF (MOF1 and MOF2) or an irrelevant control Lamin A/C (Lamin) and harvested 4 days post-transfection. Genomic DNA was isolated, modified by sodium bisulfite and amplified by methylation specific PCR (MSP) with primer sets specific for either methylated (M) or unmethylated (U) DNA. DNA from IMR90 and MCF7 cells served as a control for unmethylated DNA, while that from HMT.1E1 and MDA-MB231 cells served as a control for methylated DNA. The TMS1 region (32-223) amplified by MSP is shown in Part B (MSP). B) DNA from MCF7 cells transfected with Lamin A/C (Lamin) or MOF2 (hMOF) siRNA was modified with bisulfite and amplified with a primer set that spans 53 CpG sites in the TMS1 CpG island (27). Products were subcloned and sequenced. Each row indicates the sequence of an independent clone where methylated (black circles) and unmethylated (white circles) CpG sites are indicated.