Figure 2.

BCR overexpression blocks the formation of Bcr-Ablinduced solid tumors in nude mice. (a) A total of 107 cells of each cell line were injected subcutaneously into the flanks of nude mice that were given water with of without 800 μg ml⁻¹ doxycycline 1 week before injections, and during the whole duration of the experiment. We injected 10 mice (20 injection sites) for the TonB210/GFP+doxy and for TonB210/BCR/GFP+doxy groups, and five mice (10 injection sites) for the same cell lines, but in the −doxy conditions. Number of tumors was counted and expressed as a percentage of the total number of injected sites. An insignificant number of Bcr-Abl-independent tumors were also observed in the control group TonB210/GFP−doxy. (b) The diameter of tumors was measured by using a caliper at day 16 post injection and at day 23 when the animals were killed. Comparison analysis of tumor diameters between the two groups showed significant differences with P-values of 2.26449E-05 and 0.01513 at days 16 and 23, respectively. (c) After the animals were euthanized, tumors were isolated and immediately weighed. Comparison analysis of tumor weights for the two groups showed a significant difference between TonB210/GFP+doxy and TonB210/BCR/GFP+doxy (P-value= 0.008525). doxy, doxycycline; GFP, green fluorescent protein.