Kinase domain BCR mutants S354A and Y360F enhance the formation of Bcr-Abl-induced solid tumors in nude mice. (a) Conditions are the same as described in Figure 2. Ten animals were used for TonB210/BCRS354A/GFP and TonB210/BCRY360F/GFP cells. Seven and eight animals were used for TonB210/GFP and TonB210/BCR/GFP, respectively. Mice injected with TonB210/BCRY360F/GFP cells were euthanized at day 12 post injection; the other groups were killed between days 21 and 22 post injection. (b) Tumor diameters were measured as in Figure 2. Significant differences were observed between TonB210/GFP and TonB210/BCR/GFP (P-value = 0.00214), TonB210/BCR/GFP and TonB210/BCRS354A (P-value = 0.00029), and TonB210/BCR/GFP and TonB210/BCRY360F (P-value = 0.00199). (c) Tumor weights were measured as in Figure 2. Significant differences are observed between TonB210/GFP and TonB210/BCR/GFP (P-value = 0.00429), TonB210/BCR/GFP and TonB210/BCRS354A/GFP (P-value = 0.00321), and TonB210/BCR/GFP and TonB210/BCRY360F/GFP (P-value = 0.00022). GFP, green fluorescent protein.