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Wild-type Bcr but not kinase domain-mutant BCR proteins blocks the formation of colonies in a soft agar. (a) Thirty thousand cells were seeded in four different plates for each cell line. The assay was repeated four times for TonB210/GFP and TonB210/BCR/GFP and two times for TonB210/BCRS354A/GFP and TonB210/BCRY360F/GFP. (b) Colonies were counted after staining with p-iodonitrotetrazolium violet 14 days post inoculation. GFP, green fluorescent protein.
