Figure 5. Response of Cutl2, Trim24 and Tox RNAs to continuous GH infusion in mice.

Male ICR mice were implanted with osmotic mini-pumps delivering a continuous infusion of GH or vehicle control for time periods ranging from 10 h to 14 d. Individual livers were isolated and analyzed for Cutl2, Trim24 and Tox RNAs by qPCR. Data shown are based on the following number of individual mice per group: n=6–7 (each GH-treated time point) and n=2–3 (each vehicle-treated control group; sham). RNA levels (mean ± SE) were normalized to the 18S rRNA content of each liver and are presented relative to the average untreated female liver level, which was set to 1. All three RNAs showed stress-dependent responses to osmotic mini-pump implantation at the 10 h time point, as indicated by RNA increases in the vehicle-treated control group. The stress response of Tox RNA, evident at several of the early time points, was quite substantial and was indistinguishable for vehicle control and GH-treated livers at the 10h point (increase up to 80–95% of the untreated female control for both groups). Tox RNA data for the 10 h time point (arrow) was adjusted for the stress response by multiplying the relative expression levels in GH- and vehicle-receiving groups by the untreated male/vehicle control expression ratio.