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. 2008 Dec 5;4(12):e1000291. doi: 10.1371/journal.pgen.1000291

Figure 1. Transformation construct and expression of I-PpoI in the testes of transgenic mosquitoes.

Figure 1

(A) Schematic representation of the construct pBac{3xP3-DsRed}β2-eGFP::I-PpoI containing the left and right piggyBac inverted repeats (pBacR,L); the Pax promoter (3xP3) driving the DsRed marker gene; and the eGFP::I-PpoI effector gene (eGFP I-PpoI) under the control of β2 tubulin promoter and terminator (β2). (B) Transmission and fluorescent images of dissected adult testes, larval head and pupa of β2Ppo1 male mosquitoes. (C) Southern blot analysis of the 28S ribosomal DNA locus. DNA from testes of WT (lanes 1 and 3) and β2Ppo1 males (lanes 2 and 4) was digested with the endonuclease ClaI in vitro and hybridized with a probe encompassing the 28S ribosomal gene (Figure S1). As control both the DNA extracted from the WT and β2Ppo1 testes was treated with recombinant I-PpoI as indicated. Furthermore the PCR product (2kb) used as probe either treated with recombinant I-PpoI or untreated was analysed under the same hybridization conditions (lanes 4 and 5). Open and filled arrowheads indicate the full length and digested rDNA fragments respectively.