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. 2008 Nov 24;205(12):2915–2927. doi: 10.1084/jem.20080174

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© 2008 Ishimaru et al.

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Figure 3. — Antigen-presenting function in salivary epithelial cells. (A) MHC class II expression of salivary tissues from WT and RbAp48-Tg mice was detected by confocal analysis using anti–MHC class II mAb, Alexa Fluor 568–conjugated anti–rat IgG (red) and DAPI (blue). Images are representative of five to seven mice. (B) APC markers of splenocytes, splenic DCs, and MSG cells from WT and RbAp48-Tg mice were analyzed by flow cytometer. Results are representative of three to five mice in two independent experiments. (C) APC markers of MSG epithelial cells were detected by confocal analysis using anti-MHC class II, CD86, CD80, ICAM-1 mAbs, anti-keratin polyclonal antibody, Alexa Fluor 568–conjugated anti–rat IgG (red), Alexa Fluor 488–conjugated anti–rabbit IgG (green), and DAPI (blue). Images are representative of five to seven mice. Bars: (A and C) 50 μm.