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. 2008 Dec;132(6):731–744. doi: 10.1085/jgp.200810108

Figure 2.

Figure 2.

VG currents in taste cells dialyzed and perfused with MgATP+Mg(OH)2. (A) Representative WC currents (n = 4) recorded from the same cell perfused with 50 mM MgATP+Mg(OH)2 (middle) and with 12.5 mM MgATP+Mg(OH)2 (bottom). The currents were recorded with 50 mM MgATP+Mg(OH)2 in the recording pipette. Taste cells were patched in the bath solution containing 140 mM NaCl that was substituted for 50 mM MgATP+Mg(OH)2 after obtaining the WC mode. (B) Voltage current curves generated with 50 mM (•) and 12.5 mM (○) MgATP+Mg(OH)2 in the bath. With 50 mM MgATP+Mg(OH)2 both in the recording pipette and in the bath, WC currents did not reverse at 0 mV, as membrane voltage was not corrected for the liquid junction potential of ∼10 mV between solutions containing 140 mM NaCl and 50 mM MgATP+Mg(OH)2, respectively. Nearly the same junctional potential arose across the boundary between the solutions containing 140 mM NaCl and 12.5 mM MgATP+Mg(OH)2. Because the lifetime of stable WC preparations was very short with high MgATP+Mg(OH)2 in the pipette, neither serial resistance nor cell capacitance was compensated for in the presented recordings.