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. 2008 Dec;132(6):731–744. doi: 10.1085/jgp.200810108

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© 2008 Romanov et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — ATP release on electrical stimulation is not accompanied by a change in intracellular Ca²⁺. (A and B) Representative simultaneous recordings of ionic currents and fluorescence in a type III cell loaded with 4 μM Fluo-4 (n = 6). The depolarization of the taste cell to −10 mV for 100 ms (A and B, top panels) elicited the VG Ca²⁺ current (A, bottom panel, dotted line) that produced the Ca²⁺ transient monitored as an increase in Fluo-4 fluorescence (B, bottom panel). A relative change in fluorescence intensity is expressed as ΔF/F₀, where F₀ is a value of Fluo-4 emission measured just before cell stimulation. (C and D) Representative concurrent recordings of ionic currents (middle panel) and Fluo-4 fluorescence (bottom panel) in a type II cell (n = 21). Unlike ionomycin, 2-s depolarization (top panel) did not affect intracellular Ca²⁺ in 17 of 21 type II cells (C). In 4 out of 21 cells, depolarization induced a small increase in Fluo-4 fluorescence (D). (E) Representative concurrent recordings of a membrane current and intracellular Ca²⁺ in a type II cell and an ATP sensor response (n = 9). The 5-s depolarization (top panel) of the taste cell produced no change in cytosolic Ca²⁺ (the second trace from the bottom) but triggered ATP release, thereby stimulating the ATP sensor (bottom panel). The recording conditions were as in Fig. 1.