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. 2008 Dec;132(6):731–744. doi: 10.1085/jgp.200810108

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© 2008 Romanov et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Dependence of ATP secretion on membrane voltage. (A and B) Calculated quantity of ATP released on 2-s (A) and 100-ms (B) electrical stimulations. The thick lines depict the total ATP quantity versus membrane voltage calculated as Q_ATP = Q_a + Q_d using Eqs. 11, 12, and 14 with the following parameters: G₀ = 1, r = 0, V₀ = RT/zF = 13 mV at z = 2, V_g and V_g0 as in Eq. 16, and with τ_a and τ_d determined by Eqs. 18 and 19, respectively. The dotted and dashed curves correspond to Eqs. 11 and 12, respectively, at the parameters mentioned above. The thin curve describes Q_ATP = Q_a + Q_d calculated with z = 4 and the other parameters as above. Each dependence in A and B was normalized to the maximal value of Q_ATP. (C) Normalized response of the ATP sensor (•) versus voltage clamped on the plasma membrane of an assayed taste cell. The ATP responses were recorded at 2 s of depolarization of taste cells. The thick and thin curves were obtained by converting Q_ATP calculated at z = 2 and z = 4 (thick and thin curves in A, respectively) into ATP sensor responses using Eq. 20 with = 0.49. Both experimental and simulated responses were normalized to the corresponding maximal value. (D) ATP responses (▾) recorded at the serial stimulation of taste cells by 100-ms pulses for 2 s. The thick and thin curves were obtained by converting Q_ATP calculated at z = 2 and z = 4 (thick and thin curves in B) into ATP sensor responses using Eq. 20 with = 0.49. The responses were normalized to a value of the maximal response. The experimental data are presented as a mean ± SD (n = 5–8). The recording conditions were as in Fig. 1.

Inline graphic — Dependence of ATP secretion on membrane voltage. (A and B) Calculated quantity of ATP released on 2-s (A) and 100-ms (B) electrical stimulations. The thick lines depict the total ATP quantity versus membrane voltage calculated as Q_ATP = Q_a + Q_d using Eqs. 11, 12, and 14 with the following parameters: G₀ = 1, r = 0, V₀ = RT/zF = 13 mV at z = 2, V_g and V_g0 as in Eq. 16, and with τ_a and τ_d determined by Eqs. 18 and 19, respectively. The dotted and dashed curves correspond to Eqs. 11 and 12, respectively, at the parameters mentioned above. The thin curve describes Q_ATP = Q_a + Q_d calculated with z = 4 and the other parameters as above. Each dependence in A and B was normalized to the maximal value of Q_ATP. (C) Normalized response of the ATP sensor (•) versus voltage clamped on the plasma membrane of an assayed taste cell. The ATP responses were recorded at 2 s of depolarization of taste cells. The thick and thin curves were obtained by converting Q_ATP calculated at z = 2 and z = 4 (thick and thin curves in A, respectively) into ATP sensor responses using Eq. 20 with = 0.49. Both experimental and simulated responses were normalized to the corresponding maximal value. (D) ATP responses (▾) recorded at the serial stimulation of taste cells by 100-ms pulses for 2 s. The thick and thin curves were obtained by converting Q_ATP calculated at z = 2 and z = 4 (thick and thin curves in B) into ATP sensor responses using Eq. 20 with = 0.49. The responses were normalized to a value of the maximal response. The experimental data are presented as a mean ± SD (n = 5–8). The recording conditions were as in Fig. 1.