Display of surface proteins by staphylococci. Microtiter dishes were coated with increasing amounts of Ig, fibronectin, or fibrinogen. Binding of staphylococci to mammalian proteins was detected by staining with crystal violet and measuring the absorbance at 570 nm in a spectrophotometer. (A) Binding of S. aureus RN4220 (♦), OS2 (spa−, ▴), and SKM1 (srtA−, ■) to Ig. (B) Binding of S. aureus RN4220 (♦), and SKM1 (srtA−, ■) to fibronectin. (C) Binding of S. aureus Newman (♦) and SKM3 (srtA−, ■) to fibrinogen.