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. Author manuscript; available in PMC: 2009 Oct 15.

Published in final edited form as: Toxicol Appl Pharmacol. 2008 Jul 15;232(2):337–350. doi: 10.1016/j.taap.2008.07.004

Effects of orally administered isopimpinellin on hepatic expression of Cyp2b10 and Cyp3a11 in CAR(+/+) wild type vs. CAR(−/− knockout mice. Mice were treated with a single dose of isopimpinellin (150 mg/kg, per os) and sacrificed 24 h later. Total liver RNA was analyzed by Northern blot (Panel A) for Cyp2b10 and Cyp3a11. First lane: Co, corn oil control; Lanes 2–4: isop1–3, isopimpinellin, 3 individual mice; Lane 5: TC, mice were treated with a known inducer, TC (3 mg per kg body weight, i.p., 6 h). Panel B, densitometry analysis of the effects of isopimpinellin on Cyp2b10 and Cyp3a11. Individual values (both male and female) were pooled together and the data are expressed as a percentage of the control (means ± SD or range: corn oil control, n=2; isop. n=6; TC, n=2). Bars with the superscript a were significantly different from each other (p ≤0.05).

Effects of orally administered isopimpinellin on hepatic expression of Cyp2b10 and Cyp3a11 in CAR(+/+) wild type vs. CAR(−/− knockout mice. Mice were treated with a single dose of isopimpinellin (150 mg/kg, per os) and sacrificed 24 h later. Total liver RNA was analyzed by Northern blot (Panel A) for Cyp2b10 and Cyp3a11. First lane: Co, corn oil control; Lanes 2–4: isop1–3, isopimpinellin, 3 individual mice; Lane 5: TC, mice were treated with a known inducer, TC (3 mg per kg body weight, i.p., 6 h). Panel B, densitometry analysis of the effects of isopimpinellin on Cyp2b10 and Cyp3a11. Individual values (both male and female) were pooled together and the data are expressed as a percentage of the control (means ± SD or range: corn oil control, n=2; isop. n=6; TC, n=2). Bars with the superscript a were significantly different from each other (p ≤0.05).