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. Author manuscript; available in PMC: 2008 Nov 21.
Published in final edited form as: Vaccine. 2008 Jul 25;26(38):4927–4939. doi: 10.1016/j.vaccine.2008.07.015

Fig. 5.

Fig. 5

Antibodies against individual spore proteins do not block germination. Heat-inactivated Sterne spores (1 × 108) were pre-incubated with anti-spore serum 311001-01 (□), anti-BclA (○), anti-BxpB (▲), anti-p5303 (×), or NRS (◆) before inoculation of 5% BHI broth. Colony counts of heat-treated samples that were taken from the cultures at various time points (tx) were plotted as log10 cfu against each time point. Error bars represent ±1 standard deviation from three separate experiments. Colony counts following heat-treatment represent ungerminated spores. *The level of germination of spores pre-incubated with 311001-01 was statistically lower than wild type at 15 min (P = 0.048) and 30 min (P = 0.019).