Table 1.

B. anthracis genes cloned and expressed in this study

Sterne locus	Ames locus	Function	ORF (bp)	Protein size (kDa)	Anti-spore reactivity (31101-01)	Reference
BAS0108	BA0108	Translation elongation factor Tu	1188	42.9	−	[43,44]
BAS0238	BA0252	Alanine racemase	1170	43.6	−	[32,43–45]
BAS0253	BA0267	GroEL	1635	57.4	−	[43,46]
BAS0340	BA0355	CotB homolog	522	19.4	−	[43]
BAS0766	BA0803	CotJC	570	21.6	−	[44,47]
BAS0767	BA0804	CotJB	276	10.9	−	[44]
BAS0768	BA0805	CotJA	216	8.4	−	[44]
BAS1130	BA1222	BclA	1203	36.8	+	[32,33]
BAS1141	BA1234	CotZ1	459	16.1	+	[43,44]
BAS1144	BA1237	BxpB	504	17.3	+	[32,43,44]
BAS1145	BA1238	CotZ2	471	16.8	−	[43,44]
BAS1378	BA1489	Fe–Mn superoxide dismutase (SOD15)	915	36.1	+	[32,44,45]
Δ1–96 BAS1378	Δ1–96 BA1489	Truncated Fe–Mn superoxide dismutase (SOD15)	627	24.6	+	[32,45]
BAS1655	BA1786	ExsE	957	35.8	−	[48]
BAS2008	BA2162	BxpA	705	27.7	−	[32,44]
BAS2138	BA2292	Hypothetical protein (p2138)	756	28.5	−	[44]
BA2150 homolog	BA2150	ExsG	153	5.4	−	[48]
BAS2174	BA2332	BxpC	399	14.4	−	[43]
BAS2377	BA2554	Hypothetical protein (p2377)	330	12	+	[43,44]
BAS2439	BA2617	ExsD	465	17.4	+	[48]
BAS2693	BA2888	Inosine-uridine preferring nucleoside hydrolase	969	36.3	−	[47]
BAS2986	BA3211	Hypothetical protein (p2986)	474	17.8	−	[47]
BAS3402	BA3668	Glycosyl hydrolase, family 18	1293	48.1	−	[44]
BAS3619	BA3906	CotE	543	20.4	−	[48]
BAS3957	BA4266	Hypothetical protein (p3957)	363	13.4	+	[44]
BAS4177	BA4499	Mn superoxide dismutase (SODA1)	612	22.6	+	[44]
BAS4383	BA4722	ThiJ/PfpI family protein	663	24	+	[44]
BAS4544	BA4898	Small, acid-soluble spore protein B	198	6.8	−	[44]
BAS5241	BA5640	Cell wall hydrolase	423	16.1	−	[44]
BAS5242	BA5641	YwdL	438	16.2	+	[44]
BAS5303	BA5699	Hypothetical protein (p5303)	402	15	+	[44]