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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Exp Physiol. 2008 Feb 22;93(7):817–824. doi: 10.1113/expphysiol.2008.042085

Figure 3. Rat kidney immunostaining of glomeruli and tubules in adrenalectomized normoglycaemic, adrenalectomized diabetic adrenalectomized diabetic rats treated with FAD286.

Figure 3

Immunoglobulin G immunostaining was used as negative control for TGFβ (A and E), fibronectin (I) and collagen type IV (M) in normoglycaemic adrenalectomized rats. Renal immunostaining (brown) in normoglycaemic adrenalectomized rats is presented for TGFβ (B and F), fibronectin (J), collagen type IV (N) and PAS (Q). Renal immunostaining (brown) is increased in diabetic adrenalectomized rats for TGFβ (C and G), fibronectin (K), collagen type IV (O) and PAS (red staining, R). Treatment of adrenalectomized diabetic rats with the aldosterone synthase inhibitor FAD286 reduced renal immunostaining (brown) for TGFβ (D and H), fibronectin (L), collagen type IV (P) and PAS (red staining, S). Scale bars represent 50 μm.