Figure 10.

Measurement of apoptosis by TUNEL staining of HMVEC-L infected with HHV-8 as compared with cells exposed to conditioned media (CM) and untreated cells. The HMVEC-L were infected via co-culture with BCBL-1 cells. The cells were assayed at two time points (Day 3 and Day 20) after infection or exposure to CM. At these time points the cells were exposed to camptothecin for 4 hours to induce apoptosis, and then TUNEL staining was performed. The percent of TUNEL-positive cells were quantified by light microscopy and compared between the three groups at each time point. Three separate infections and three controls were performed for each time point. The first row represents untreated cells at (a) Day 3 and (b) Day 20. The second row demonstrates cells exposed to CM at (c) Day 3 and (d) Day 20. The third row demonstrates cells infected with HHV-8 via co-culture at (e) Day 3 and (f) Day 20. Figures are at ×40 magnification. The percent of TUNEL-positive cells are represented graphically below the figures. *P < 0.05.