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q-PCR assay for markers of viral infection in HHV-8–infected HMVEC-L, mock-infected cells (negative control), and BCBL-1 cells (positive control). The genes assayed by PCR were T1.1, K8.1, LANA-1, ORF-50, and vGPCR. Both HHV-8–infected HMVEC-L and BCBL-1 cells were positive for HHV-8 gene expression indicating infection. Mock-infected cells were negative for PCR evidence of HHV-8 infection.
