Figure 5.

q-PCR analysis of genes selected for confirmation of the microarray results. The gene expression of HMVEC-L infected with HHV-8 via direct virus exposure was compared with that of mock-infected cells (see Materials and Methods). Gene expression was assayed 72 hours after exposure. All infections and controls were performed in triplicate. Error bars represent SEM. As predicted by microarray analysis, there was decreased expression of BMP-4, BMPR1a, TGFB-2 and increased expression of Noggin, IL-6, MMP-1, and FLT-1 in cells infected with HHV-8 as compared with mock-infected cells.^*P < 0.05.