Figure 9.

Measurement of apoptosis by caspase 3/7 assay in HMVEC-L infected with HHV-8 as compared with cells exposed to conditioned media (CM) and untreated cells. The HMVEC-L were infected via co-culture with BCBL-1 cells. The cells were assessed at three time points after infection: (a) Day 10, (b) Day 15, and (c) Day 20. On the day of the selected time point the cells were exposed to camptothecin for 4 hours to induce apoptosis. Caspase 3/7 levels were then measured via luminescence. There were five replicate wells for each condition (n = 5). IFAs were performed to confirm HHV-8 infection of the HMVEC-L cells and lack of infection in the cells exposed to CM. Approximately 10 to 20% of the cells were HHV-8 infected (LANA-1 positive). *P < 0.05.