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. Author manuscript; available in PMC: 2009 Nov 12.
Published in final edited form as: FEBS Lett. 2008 Oct 16;582(27):3744–3750. doi: 10.1016/j.febslet.2008.10.008

Fig. 2. Gene fusion analysis of cAMP-CRP regulation of cqsA expression.

Fig. 2

(A). Transcriptional fusion. β-Galactosidase expression in strains C72ZTC (WT background) and WL72ZTC (Δcrp background) containing a cqsA-lacZ transcriptional fusion. Strains were grown at 37°C with agitation in LB overnight. (B). Translational fusions. β-Galactosidase expression in strains C7258ΔlacZ (WT background) and WL7258ΔlacZ (Δcrp background) containing the cqsA-lacZ translational fusions pVIK1847 (open bar) or pVIK1648 (shadowed bar). Strains were grown at 37°C with agitation in LB overnight. (C). Ectopic expression of cqsA. Strains AJB61 (C7258ΔcqsA) and WL7258 (C7258Δcrp) containing pTac-CqsA were grown at 37°C with agitation in TSB overnight and production of CAI-1 activity was determined as described in methods. In all cases, each value represents the average of three independent experiments. Error bars indicate standard deviations.