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. Author manuscript; available in PMC: 2009 Nov 12.
Published in final edited form as: FEBS Lett. 2008 Oct 16;582(27):3744–3750. doi: 10.1016/j.febslet.2008.10.008

Fig. 4. Longevity of cqsA mRNA in WT, Δcrp and Δcya genetic backgrounds.

Fig. 4

(A). Overnight cultures of WL7258 ( crp) and AJB61 (ΔcqsA) containing pBRcqsA2383 were diluted 100-fold in fresh TSB and grown at 37°C with agitation to OD600 1.0. Rifampicin was added and samples were taken at the indicated time points for Northern blots using a DIG-labeled cqsA probe. Each lane contains 25-μg of total RNA. (B). Overnight cultures of WL7259 (C7258Δcya) were diluted 100-fold in fresh TSB and grown at 37°C with agitation to OD600 0.5. Each culture was divided in halves, one half was used as a control and the other half was transferred to a new flask containing cAMP (5-mM). The cultures were incubated 1-h as described above and rifampicin was added to block transcription. Samples were taken at the indicated time points for Northern blot analysis. Each lane was loaded with 25-μg of total RNA. After stripping the cqsA probe, the membrane was re-probed with a DIG-labeled recA DNA fragment.