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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Structure. 2008 Oct 8;16(10):1491–1502. doi: 10.1016/j.str.2008.06.014

Figure 4. Pseudoatomic model of the EI-I/II particle.

Figure 4

(A) 3.4 Å Head-II subunit structures (backbone trace shown; A-C subunits orange, D-F subunits blue, G subunits red) were modeled as rigid bodies into a 14 Å cryo-EM reconstruction of minimally-crosslinked, WT EI-I particles (grey mesh). (B) Cross-sectional view of the pseudoatomic model fit into the EM density map. (C) Exterior view down a 5-fold axis of symmetry. In the model shown, the E-loops are in the crosslinked, down conformation found in Head-II (solid arrow), whereas the EM density for the un-crosslinked EI-I particles shows nubs of density that project outward from the surface for the E-loops in the uncrosslinked, up conformation (open arrow).