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. Author manuscript; available in PMC: 2008 Nov 24.
Published in final edited form as: J AOAC Int. 2004;87(5):1070–1082.

Table 5.

Peak area ratios of β-carotene isomers in eluents of LC-systems A and B of the present method compared with earlier LC system (25)

Eluent of LC system published earliera 445 nm, 0.8 mL/min
Eluent of LC system A, 448 nm, 0.6 mL/min
Sample Peak areasb cis/trans ratio Peak areasb cis/trans ratio
All-trans-β-carotene 1209 ± 21 – 902 ± 15 –
9-cis-β-carotene 1241 ± 4 1.03 925 ± 7 1.03
13-cis-β-carotene 375 ± 19 0.31 281 ± 9 0.31
15-cis-β-carotene 774 ± 25 0.64 573 ± 8 0.64
Eluent of LC system published earliera 445 nm, 0.8 mL/min
Eluent of LC system B, 445 nm 0.4 mL/minc
Peak areasb cis/trans ratio Peak areasb cis/trans ratio
All-trans-β-carotene 546 ± 2 – 1083 ± 3 –
9-cis-β-carotene 560 ± 4 1.03 1115 ± 1 1.03
13-cis-β-carotene 447 ± 1 0.82 881 ± 4 0.81
15-cis-β-carotene 390 ± 2 0.71 774 ± 1 0.72
a

Methanol–THF(99 + 1), 100 ppm vitamin C (25).

b

Mean and standard deviation from 4 injections.

c

Flow rate was sent to a 0.4 mL/min for better separation of isomers.