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Preparation of working aflatoxin calibrant solutions
| Final aflatoxin concentration of working calibrant solution1 ng/mL |
||||||
|---|---|---|---|---|---|---|
| Working calibrant solution | 2nd Stock standard solution, μL | AFB1 | AFB2 | AFG1 | AFG2 | AF |
| 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 2 | 12.5 | 0.25 | 0.0625 | 0.125 | 0.0625 | 0.5 |
| 3 | 25 | 0.5 | 0.125 | 0.25 | 0.125 | 1 |
| 4 | 50 | 1 | 0.25 | 0.5 | 0.25 | 2 |
| 5 | 100 | 2 | 0.5 | 1 | 0.5 | 4 |
| 6 | 200 | 4 | 1 | 2 | 1 | 8 |
